Article Sidebar

Download
pdf
Statistic
Read Counter : 396 Download : 270

Downloads

Download data is not yet available.

Main Article Content

Abstract

The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing of local ram semen with different combination of BSA concentration. The research object was ten ejaculated semen of local ram, three years old. The research design used CRD (completely randomize design) with four treatments of BSA concentration combination on upper and bottom layer (T1: 3% & 6%, T2: 4% & 6%, T3: 5% & 10%, and T4: 6% & 12%), and 10 repetitions. Data analysis using analysis variance and Duncan’s Multiple Range Test. The result showed that combination of BSA concentration was significantly effect on proportion of X-Y chromosome bearing sperm of local ram sperm. The higher average proportion of X- and Y- chromosome bearing sperm was obtained at combination 5% (75.55±1.09% for X) at upper layer and 10% BSA (76.45±1.12% for Y) of bottom layer. The conclusion is that combination of BSA concentration significantly effect on proportion of X-Y chromosome bearing sperm, and concentration of 5% and 10% BSA at upper layer and bottom layer gave the higher proportion of X-Y chromosome bearing sperm of local ram sperm.

Keywords

X-Y chromosom bearing sperm bovine serum albumin local ram Sperma yang mengandung kromosom X-Y domba lokal jantan

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Submission of a manuscript implies that the work described has not been published before or is under consideration for publication elsewhere (except in the form of an abstract). When the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.

How to Cite
Solihati, N., Rasad, S. D., Hilmia, N., Winangun, K., & Toha, T. (2023). X-Y Chromosom bearing Sperm Proportion of Local Ram after Sexing with Different Combination of Bovine Serum Albumin (BSA) Concentration. ANIMAL PRODUCTION, 25(1), 8-13. https://doi.org/10.20884/1.jap.2023.25.1.137
Crossref
Scopus
Google Scholar
Europe PMC

References

  1. Arifiantini, RI. 2012. Teknik Koleksi dan Evaluasi Semen Pada Ternak. IPB Pres. Bogor.
  2. Chen, L, Y Ge, Y Liang, and B Yao. 2014. Semen pH effects sperm motility and capacitation by influ-encing na/k-atpase activity and ca concentration in sper-moplasm. Transl. Androl. Urol. 3 (Suppl 1): AB185.
  3. Contri, A, A Gloria, D Robbe, C Valorz, L Wegher, and A Carluccio. 2013. Kinematic study on the effect of pH on bull sperm function. J. Anim. Reprod. Sci. 136 (4): 252-259.
  4. Dethan, AA, Kustoo, and H Hartadi. 2010. Kualitas dan kuantitas sperma kambing bligon jantan yang diberi pakan rumput gajah dengan suplementasi tepung darah. Buletin Peternakan. Vol. 34 (3):145-153
  5. Garner, DL. 2006. Flow cytometric sexing of mammalian sperm. Theriogenology. 65: 943–957.
  6. Herdis, IWA Darmawan, and Nuradianto. 2015. Peranan Dextrosa dalam Mempertahankan Daya Hidup Spermatozoa dalam Proses Pembekuan Semen Domba. In: Proceeding Seminar Nasional Bioresources Untuk Pembangunan Ekonomi Hijau. Peran Bioteknologi dalam Peningkatan Populasi dan Mutu Genetik Ternak Mendukung Kemandirian Daging dan Susu Nasional. Pp: 256- 265.
  7. Johnson, LA. 2000. Sexing Mammalian Sperm for Production Offspring: The State of-the-art. Anim. Reprod. Sci. 61: 93– 107.
  8. Kaiin, EM, F Afiati, M Gunawan, and B Tappa. 2003. Pengaruh Penyimpanan pada Temperatur 5oc terhadap Viabilitas Spermatozoa Sapi Hasil Pemisahan. In: Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Pp: 73-76.
  9. Lu, Y, SS Lu, M Zhang, and Y Xiaogan. 2013. Sperm Sexing In Buffalo Using Flow Cytometry Buffalo Bull. 32: 22-30.
  10. Majorek, KA, PJ Porebski, A Dayal, MD Zimmerman, K Jablonska, AJ Stewart, M Chruszcz, and W Minor. 2012. Characterization Of Bovine, Horse, And Rabbit Serum Albumins. Molecullar Immunology. Vol 52 (3-4) :174-182.
  11. Maxwell, WMC, G Meddoa, and IG Shite. 1984. Post Thawing Survival of Motile Ram Sperm After Isolation by Layering on Protein Columns. Teriogenology. 21: 601-606.
  12. Meistrich, M. 1982. Potentials and Limitations of Physical Methods for Separations of Sperm Bearing An X Or Y Chromosome Prospects For Sexing Mammalian Sperm. Colorado Associated Univ. Press. Boulder, Colorado. Pp: 145-163.
  13. Nahriyanti, S, YS Ondho, and D Samsudewa. 2017. Perbedaan Kualitas Makroskopis Semen Segar Domba Batur dalam Flock Mating dan Pen Mating. J. Sain Peternakan Indonesia. Vol. 12 (2): 191-198.
  14. Nalley, WMM, and R I Arifiantini. 2013. The Hypo-osmotic Swelling Test in Fresh Garut Ram Spermatozoa. J. Indonesian Trop. Anim. Agric. Vol. 38(4): 212-216.
  15. Parrilla, I, JM Vazquez, J Roca, and EA Martinez. 2004. Flow Cytometry Identification Of X- And Y-Chromosome Bearing Goat Sperm. Reprod. Dom. Anim. 39: 58–60.
  16. Sharma, M, and N Sharma. 2016. Sperm Sexing in Animals. Advance in Animal and Veterinary Science. Vol. 4(10): 543-549.
  17. Solihati, N, SD Rasad, A Yusrina, and YI Dimyati. 2017a. Identifikasi Morfometrik Sperma Domba Lokal sebagai Dasar Aplikasi Sexing Sperma. Jurnal Ilmu Ternak. Vol. 17(2): 109-113.
  18. Solihati, N, SD Rasad, and C Alvionita. 2018c. Quality and Viability of Javanese Local Ram Semen at Different Age. Proc-Intsem.LPVT. Pp: 265-270.
  19. Solihati, N, SD Rasad, and N Hilmia. 2022. The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration. Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS). Advances in Biological Sciences Research. Vol. 20: 141-144.
  20. Solihati, N, SD Rasad, N Hilmia, K Winangun, Toha, and OV Zule. 2020. Characteristic Several Level of Bovine Serum Albumin (BSA) and Its Combination as Albumin Colums for Sperm Sexing Media. Proc. Semnas TPV.
  21. Solihati, N, SD Rasad, R Setiawan, and S Nurjanah. 2018a. Pengaruh Kadar Gliserol Terhadap Kualitas Semen Domba Lokal. Jurnal Biodjati, 3(1): 63-71.
  22. Solihati, N, SD Rasad, R Setiawan, EN Foziah, and ET Wigiyanti. 2018b. Semen Quality of Post-Thawed Local Ram's in Tris-Egg Yolk Extender with Different Glutathione Level. IOP Conference Series: Earth and Environment.
  23. Solihati, N, Soeparna, SD Rasad, and R Ferlianthi. 2017b. Proportion and Quality of X-Y Chromosome Bearing Sperm on Diluted Semen after Incubation in Different Time of Etawah Crossbreed Goat. In: Proceedings. The 7th International Seminar on Tropical Animal Production. Yogyakarta, Indonesia. Pp: 696-671.
  24. Somarny, WWMZ, YJ Tan, SM Tasol, Y Jasmi, K Musaddin, and JA Johari. 2011. Separation of Y- Chromosome Bearing Bull’s Spermatozoa Using an Albumin Gradient Technique. Malaysia Journal of Animal Science.14 (1): 13-19.
  25. Susilawati, T. 2011. Spermatozology. UB Press, Malang. Pp 14-18.
  26. Zhang, XG, GJ Yan, JY Hong, ZZ Su, GS Yang, QW Li, and JH Hu. 2015. Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17 °C. J. Reprod. Domest. Anim. 50: 263–69. doi: DOI: 10.1111/rda.12481. 

  27. Zhou, J, L Chen, J Li, H Li, Z Hong, M Xie, S Chen, and B Yao. 2015. The Semen pH Affects Sperm Motility and Capacitation. Plos One. 10 (7): e0132974.31.