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Abstract

This study aimed to determine the effectiveness of antibacterial formulations derived from herbal plants against mastitis-causing bacteria in dairy cattle. Herbal extracts composed of betel leaves extract, kecombrang flower extract, and turmeric extract were tested against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. The research was carried out in a Completely Randomized Design (CRD) using ten treatments and four replications at the Laboratory of Research and Testing, Faculty of Animal Husbandry, Padjadjaran University. The results showed that herbal antibacterial formulations could inhibit the growth of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteria. The most inhibitory power was obtained from P8 treatment (50% betel leaves + 50% kecombrang flower + 50% turmeric) categorized in "strong inhibition." The percentage of antibacterial inhibition of P8 formulation compared to chloramphenicol was 32% against Staphylococcus aureus, 33% against Streptococcus agalactiae, and 31% against Escherichia coli. Conclusively, the best herbal antibacterial formulation was a combination of 50% betel leaves extract, 50% kecombrang flower extract, and 50% turmeric extract.

Keywords

mastitis betel leaves extract kecombrang flower extract turmeric extract herbal antibacterial

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How to Cite
Suryanah, S., & Nurjannah, S. (2021). The Effectiveness of Herbal Antibacterial Formulations Against Mastitis-Causing Bacteria in Dairy Cattle. ANIMAL PRODUCTION, 23(3), 151-159. https://doi.org/10.20884/1.jap.2021.23.3.78
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References

  1. Agustin, D. 2005. The comparison of the antibacterial effect of irrigation solutions hydrogen peroxyde 3% and piper betle folium infusum 20% to bacterial mix. Dental Journal. 38(1):45-47.
  2. Ajizah, A. 2004. Sensitivitas Salmonella typhimurium terhadap Ekstrak Daun Psidium guajava L. Bioscientiae. 1(1):31-38.
  3. Beyene, T. 2016. Veterinary drug residues in food-animal products: its risk factors and potential effects on public health. Journal of Veterinary & Science Technology. 7(1):1-7.
  4. Bradley, A.J. and M.J. Green. 2001. Adaptation of Escherichia coli to the bovine mammary gland. Journal of Clinical Microbiology. 39(5):1845-1849.
  5. Caburian, A.B. and M.O. Osi. 2010. Characterization and evaluation of antimicrobial activity of the essential oil from the leaves of Piper betle L. E-International Scientific Research Journal. 2(1):2-13.
  6. Darwis, D. 2000. Teknik Dasar Laboratorium dalam Penelitian Senyawa Bahan Alam Hayati. Paper at Workshop Pengembangan Sumberdaya Manusia dalam Bidang Kimia Organik Bahan Alam Hayati. Faculty of Math and Science, Andalas University, Padang.
  7. Davis, W.W. and T.R. Stout. 1971. Disc plate method of microbiological antibiotic assay. Applied Microbiology. 22 (4):659-665.
  8. Effa and N.R. Puetri. 2015. Pengaruh Pemberian Ekstrak Daun Sirih (Piper betle L.) terhadap Pertumbuhan Staphylococcus aureus Isolat dari Penderita Faringitis. SEL. 2(2):57-65.
  9. Erskine, R.J., S. Wagner and F.J. DeGraves. 2003. Mastitis therapy and
  10. pharmacology. Veterinary Clinics of North America: Food Animal Practice. 19(1):109–138.
  11. Fitzgerald, J.R., S.R. Monday, T.J. Foster, G.A. Bohach, PJ Hartigan, WJ Meaney, and CJ Smyth. 2001. Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens. Journal of Bacteriology. 183(1):63–70.
  12. Harborne, J.B. 1998. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 3rd ed., Chapman & Hall, London.
  13. Herlina, N., N.D. Yanthi, M. Poeloengan, B. Tappa. 2013. Daya Hambat Formulasi Antibakteri Asal Mikroorganisme dan Tanaman terhadap Streptococcus agalactiae Penyebab Mastitis Pada Sapi Perah. In: Proceeding of Seminar Nasional Peternakan dan Forum Komunikasi Peternakan. Bogor, September 18-19, 2013. Pp:577-587.
  14. Hudaya, A., N. Radiastuti, D. Sukandar and I. Djajanegara. 2014. Uji Aktivitas Antibakteri Ekstak Air Bunga Kecombrang terhadap Bakteri E. coli dan S. aureus sebagai Bahan Pangan Fungsional. Alkauniyah Jurnal Biologi. 7(1):9-15.
  15. Jaafar, F.M., C.P. Osman, N.H. Ismail, and K. Awang. 2007. Analysis of essential oils of leaves, stems, flowers and rhizomes of Etlingera elatior (Jack) R. M. Smith. The Malaysian Journal of Analytical Sciences. 11(1):269-273.
  16. Jenie, B.S.L. and Kuswanto. 1994. Aktivitas antimikroba dari pigmen angkak yang diproduksi oleh Monasnrs purpuracs terhadap beberapa mikroba patogen dan perusak makanan. In: Proceeding of Pertemuan Ilmiah Tahunan Permi. Bogor, Desember 10, 1994. Pp:53-62.
  17. Jirna, I.N., N. Mastra, L.A. Wilan, I. Karta, and Burhannudin. 2017. Potency of lime (Citrus aurantifolia) as bio-disinfectant of Staphylococcus aureus. Dama International Journal of Researchers (DIJR). 2(1):63-67.
  18. Kusumaningsih, A. and T. Ariyanti. 2013. Pathogenic bacteria contamination in fresh dairy milk and its resistance to antibiotic. Berita Biologi. 12(1):9-17.
  19. Lawhavinit, O., N. Kongkathip, and B. Kongkathip. 2010. Antimicrobial activity of curcuminoids from Curcuma longa L. on pathogenic bacteria of shrimp and chicken. Kasetsart Journal-Natural Science. 44(3):364–371.
  20. Le Marechal, C., R. Thiery, E. Vautor, and Y. Le Loir. 2011. Mastitis Impact on Technological Properties of Milk and Quality of Milk Products-a Review. Dairy Science & Technology. 91:247-282.
  21. Loncaric, I., G.L. Stalder, K. Mehinagic, R. Rosengarten, F. Hoelzl, F. Knauer, and C. Walzer. 2013. Comparison of ESBL-And AmpC producing Enterobacteriaceae and Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Migratory and Resident Population of Rooks (Corvus frugigelgus) in Austria. PLOS ONE. 8(12): 1-11.
  22. Madigan, M.T. and J.M. Martinko. 2005. Brock Microbiology of Microorganisms. 11th Ed., Prentice Hall Inc, New Jersey.
  23. Muadifah, A., A.E. Putri, and N. Latifah. 2019. Aktivitas Gel Ekstrak Rimpang Kunyit (Curcuma domestica Val) terhadap Bakteri Staphylococcus aureus. Jurnal Sainhealth. 3(1):45-54.
  24. Mycek, M.J., R.A. Harvey, P.C. Champe. 2001. Pharmacology. 2nd ed., Widya Medika, Jakarta.
  25. Naufalin, R. and S.R. Herastuti. 2013. Microcapsule application of kecombrang flower extract: effects of concentration, types of fraction, pH of medium, and NaCl on microbiological
  26. properties of minced beef. Animal Production. 15(1):8-14.
  27. Naufalin, R., B.S.L. Jenie, F. Kusnandar, M. Sudarwamto, and H. Rukmini. 2005. Antibacterial activity of kecombrang flower extract toward pathogenic and food spoilage bacteria. Jurnal Teknologi dan Industri Pangan. 16(2):119-125.
  28. Navarro, P., R.M. Giner, M.C. Recio, S. Máñez, M. Cerdá-Nicolás, JL Ríos. 2001. In vivo anti-inflammatory activity of saponins from Bupleurum rotundifolium. Life Sciences. 68(10): 1199-1206.
  29. Olson, J. 2004. Clinical Pharmacology: Made Ridiculously Simple. 1st ed., EGC, Jakarta. 203 Pages.
  30. Pangemanan, A., Fatimawali, dan F. Budiarso. 2016. Uji Daya Hambat Ekstrak Rimpang Kunyit (Curcuma longa) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Pseudomonas sp. Jurnal e-Biomedik (eBm). 4(1):81-85.
  31. Pasca, C., L. Marghitas, D. Dezmirean, O. Bobis, V. Bonta, F. Chirila, I. Matei, and N. Fit. 2017. Medicinal plants based products tested on pathogens isolated from mastitis milk. Molecules. 22(9):1-16.
  32. Patil, R.S., P.M. Harale, K.V. Shivangekar, P.P. Kumbhar, and R.R. Desai. 2015. Phytochemical potential and in vitro antimicrobial activity of Piper betle Linn. leaf extracts. Journal of Chemical and Pharmaceutical Research. 7(5):1095-1101.
  33. PHAC (Public Health Agency of Canada). 2020. Guidelines for Canadian Drinking Water Quality: Guideline Technical Document-Escherichia coli. https://www.canada.ca/en/health-canada/services/publications/healthy-living/guidelines-canadian-drinking-water-quality-guideline-technical-document-escherichia-coli.html (accessed : December 04, 2020).
  34. Pisestyani, H., E. Sudarnika, R. Ramadhanita, A.Z. Ilyas, A. Wicaksono, C. Basri, A.B. Nugraha, and M.B. Sudarwanto. 2017. Teat dipping treatment after milking process to the presence of pathogenic bacteria, Staphylococcus aureus, Streptococcus agalactiae, and E. coli in dairy cattle with positive subclinical mastitis at Kunak Bogor. Jurnal Sain Veteriner. 35(1):63-70.
  35. Poeloengan, M., I. Komala, S.M. Noor, Andriani, and S.R.P. Rianti. 2006. Activity water extract, of essential oil and ethanol extraction of piper bittle leaves against bacteria isolated from sub clinical mastitis cattle. Seminar Nasional Teknologi
  36. Peternakan dan Veteriner 2006. Pp:250-255.
  37. Rahmawati, N., E. Sudjarwo, and E. Widodo. 2014. Uji Aktivitas Antibakteri Ekstrak Herbal terhadap Bakteri Escherichia coli. Jurnal Ilmu-Ilmu Peternakan. 24(3):24-31.
  38. Ramadhani, P., Erly, and Asterina. 2017. Hambat Ekstrak Etanol Rimpang Kunyit (Curcuma domestika V.) terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Kesehatan Andalas. 6(3):590-595.
  39. Rambe, D.R., Allaily, and C.I. Novita. 2019. Inhibitory analysis of green betel leaf extract (Piper betle L.) against mastitis causing microorganism. Jurnal Ilmiah Mahasiswa Pertanian. 4(2):230-236.
  40. Sachi, S., J. Ferdous, M.H .Sikder, and S.M.A.K. Hussani. 2019. Antibiotic residues in milk: past, present, and future. Journal of Advanced Veterinary and Animal Research. 6(3):315–332.
  41. Seegers, H., C. Fourichon, and F. Beaudeau. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research, Biomed Central. 34(5):475–491.
  42. Sutarti, E., S. Budiharta, and B. Sumiarto. 2003. Prevalence and factors associated with mastitis in smallholder dairy cows in District of Semarang, Central Java. Jurnal Sain Veteriner. 21(1):43-49.
  43. Syamsi, A.N., M. Pratiwi and A.P. Nugroho. 2019. Inhibition Activity of Garlic (Allium sativum) Skin Extract on Mastitis Causing Microorganism. Animal Production, 21(1):38-42.
  44. Volk, W.A., and M.F. Wheeler. 1993. Basic Microbiology. 5th ed., Erlangga, Jakarta.
  45. Wahyuni, A.E.T.H., I.W.T. Wibawan, F.H. Pasaribu, and B.P. Priosoeryanto. 2006. Distribution of serotype of Streptococcus agalactiae caused subclinical mastitis on dairy cattle in East Java, Center Java and West Java. Jurnal Veteriner. 7(1):1-8.
  46. Wibawan, I.W.T. and C.H. Leammler. 1990. Properties of group B Streptococci with Protein Surface Antigens X and R. Journal of Clinical Microbiology. 28(12): 2834-2836.