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Abstract

The study evaluated the growth performance, haematology, serum biochemistry, intestinal microbial count, and antimicrobial resistance profile of Escherichia coli (E. coli) from broiler chicks fed diets supplemented with antibiotics (neomycin, and oxytetracycline), and probiotic (Saccharomyces cerevisiae). One hundred and twenty Abor acre broiler chicks randomly alloted to four treatment groups (30 birds/group; 10 birds/replicate) were used in the 49 days study. Group one (G 1, control) were fed basal diet while G 2, 3, and 4 received basal diet containing S. cerevisiae (0.80g/kg; 108cfu/g), neomycin (0.50g/kg) and oxytetracycline (0.30g/kg), respectively. Results showed significant treatment effects on body weight, feed intake, linear body values, some haematological indices, intestinal, caecal and combined caecal and intestinal bacteria counts, diameter of E. coli inhibition zone, and mortality. Body weight and feed intake were significantly  higher in the supplemented groups. Intestinal bacterial count was highest in neomycin and control groups (5.29 ± 0.01 and 5.22 ± 0.02 Log10 cfu/ml, respectively) while S. cerevisiae and neomycin groups yielded the highest caecal, and combined caecal and intestinal bacterial counts. Eimeria Oocyst count did not differ significantly between groups. Escherichia coli from antibiotic fed groups had reduced sensitivity or were resistant to the antibiotics. It was concluded that subtherapeutic use of antibiotics as growth promoters in broiler chickens caused the development of antibiotic resistance, and therefore, should be avoided.

Keywords

antimicrobial resistance growth performance neomycin oxytetracycline S. cerevisiae

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How to Cite
Ogbu, C. C., & Ibeneme, N. (2021). Comparative Antibiotic and Probiotic Effects on Antimicrobial Sensitivity of Escherichia coli Isolates and Performance of Broiler Chickens. ANIMAL PRODUCTION, 22(3), 126-136. https://doi.org/10.20884/1.jap.2020.22.3.60
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References

  1. Albokhadaim I., T. Althnaian and S.M. El-Bahr. 2012 Investigation of selected biochemical parameters of local chickens with different age and sex in Al-ahsa, Saudi Arabia. Pakistan Journal of Biological Sciences 15 (17): 827-832.
  2. Ashayerizadeh A., N. Dabiri, O. Ashayerizadeh, K.H. Mirzadeh, H. Roshanfekr and M. Mamooee. 2009 Effect of dietry antibiotic, probiotic and prebiotic as growth promoters on growth performance, carcass characteristics and haematological indices of broiler chickens. Pakistan Journal of Biological Sciences 12 (1): 52-57.
  3. Beski S.S.M., S.Y.T. Al-Sardary. 2015 Effects of dietary supplementation of probiotic and symbiotic on broiler chicken haematology, and intestinal integrity. Int J Poult Sci 14 (1): 31-36.
  4. Bonsu F.R.K., S.A. Osei, D.B. Okai, J. Baah. 2012 Effect of direct-fed microbials and antibiotics supplementation on the health status and growth performance of broiler chickens under hot humid environmental conditions. Int J of Lsk Prod 3 (6): 66-71.
  5. Campos J.L., M.G. Gil, S.G. Davila, I. Munoz. 2006 The genetics of three welfare indicators: tonic immobility duration, heterophil to lymphocyte ratio, and fluctuating asymmetry. WPS J, suppl. 62: 606-611.
  6. Cheesbrough M. 1998 District Laboratory Practice in Tropical Countries, part 1. Cambridge University Press.
  7. CLSI. (2013). Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Third Informational Supplement. CLSI Document M100-S22. Clinical and Laboratory Standards Institute, Wayne, PA.
  8. Corduk M., N. Ceylan, N. Dede and O.Y. Tel. 2008 Effects of novel feed additives on performance, carcass traits and E. coli, aerobic bacteria and yeast counts in broilers. Arch. Geflugelk 72 (2). S. 61-67.
  9. Cosby D.E., N.A. Cox, M.A. Harrison, J.L. Wilson, R.J. Buhr, P.J. Fedorka-Cray. 2015 Salmonella and antimicrobial resistance in broilers: A review. J Appl Poult Res 24: 408-426.
  10. Cruickshank R.J., B.P. Marion, R.H.A. Jwan. 1975 Centrifuges, calorimeters, bacteria counts in medical microbiology 12th ed. Pp. 301-307.
  11. Diarra M.S., F. Malouin. 2014 Antibiotics in Canada poultry production and anticipated alternatives. Front Microbiol 5: 1-15.
  12. Diarra M.S., F.G. Silversides, F. Diarrassouba, J. Pritchard, L. Masson, R. Brousseau, C. Bonnet, P. Delaquis, S. Bach, B.J. Skura, E. Topp. 2007 Impact of feed supplementation with antimicrobial agents on growth performance of broiler chickens, Clostridium perfringes and enterococcus counts, and antibiotic resistance phenotypes and distribution of antimicrobial resistance determinants in Escherichia coli isolates. Appl Envt Microbiol 73 (20): 6566-6576.
  13. El-Hammady H.Y., M. El-Sagheer, H.H.M. Hassanien, H.A. Hassan. 2014 Performance and carcass traits of broilers supplemented with probiotic and neomycin antibiotic. Egypt J Anim Prod 51 (2): 107-114.
  14. Elsayed M., A. Elkomy, M. Aboubakr and M. Morad. 2014 Tissue residues, haematological and biochemical effects of tilmicosin in broiler chicken. Veterinary Medicine International. Doi: org/10: 1155/2014/502892.
  15. Fairchild A.S., J.L. Smith, U. Idris, J. Lu, S. Sanchez, L.B. Purvis, C. Hofacre, M.D. Lee. 2005 Effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and Campylobacter jejuni. Appl Envt Microbiol 71 (10): 5865-5872.
  16. Giggs J.P., J.P. Jacobs. 2005 Alternatives to antibiotics for organic poultry production. The J Appl Poult Product 14: 150-156.
  17. Gunal M., G. Yayli, O. Kaya, N. Karahan, O. Sulak. 2006 The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int J Poult Sci 5: 149-155.
  18. Gunnison J.B., E. Kunishige, V.R. Coleman, E. Jawetz. 1955 The mode of action of antibiotic synergism and antagonism: the effect in vivo on bacteria not actively multiplying. J Gen Microbiol 13: 509-518.
  19. He T., S. Long, S. Mahfuz, D. Wu, X. Wang, X. Wei and X. Piao. 2019 Effects of probiotics as antibiotics substitutes on growth performance, serum biochemical parameters, intestinal morphology, and barrier function of broilers. Animal 9: 985; doi: 10.3390/ani9110985.
  20. Islam M.W., M.M. Rahmen, S.M.L. Kabir, S.M. .Kamruzzan, S.N. Islam. 2004 Effects of probiotics supplementation on growth performance and certain haemato-biochemical parameters in broiler chickens. Bangla J Vet Med 2 (1): 39-43.
  21. Jadhav K., K.S. Sharma, S. Katoch, V.K. Sharma and B.G. Mane. 2015 Probiotics in broiler poultry feeds: A review. Journal of Animal Nutrition and Physiology 1: 4-16.
  22. Kalghatgi S., C.S. Spina, J.C. Costello, M. Liesa, J.R. Morones-Ramirez, S. Slomovic, A. Molina, O.S. Shirihai and J.J. Collins. Bactericidal antibiotics induce mitochondria dysfunction and oxidative damage in mammalian cells. Sci Transl Med. 5 (192): 192ra85. Doi: 10.11261scitranslmed.3006055.
  23. Koc F., H. Samil, A. Okur, M. Ozduven, H. Akyurek, N. Senkoylu. 2010 Effect of Saccharomyces cerevisiae and/or mannanoligosaccharides on performance, blood parameters and intestinal microbiota of broiler chicks. Bulg J Agric Sci 16: 643-650.
  24. Kumar S., C. Chen, N. Indugu, G.O. Werlong, M. Singh, W.K. Kim, H. Thippareddi. 2018. Effect of antibiotic withdrawal in feed on chicken gut microbial dynamics, immunity, growth performance and prevalence of foodborne pathogens. PloS ONE 13 (2): e0192450.
  25. Lee J.W., J.G. Shin, E.H. Hee, H.E. Kang, I.B. Yim, J.Y. Kim, H.G. Joo, H.J. Woo. 2004 Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus and Bifidobacterium longum. J Vet Sci 5 (1): 41-48.
  26. Mohamed M.A., E.F. El-Daly, N.A. Abdel Azeem, A.W. Youssef, H.M.A. Hassan. 2014 Growth performance and histological changes in ileum and immune related organs of broilers fed organic acid or antibiotic growth promoters. Int J Poult Sci 13 (10): 602-614.
  27. Neveling D.P., L. van Emmenes, J.J. Ahire, E. Pieterse, C. Smith, and L.M.T. Dicks. 2017 Safety assessment of antibiotic and probiotic feed additives for Gallus gallus domesticus. Scientific Reports 7. Doi 10.1038/s41598-017-12866-7.
  28. Odefemi T.R. 2016 Performance response and carcass characteristics of broilers fed dietary antibiotics, probiotics and prebiotics. European Journal of Agriculture and Forestry Research 4 (1): 27-36.
  29. Ologhobo A.D., L.C. Adighibe, O.O. Adeleye, I.O. Adejumo. 2015 Impact of Saccharomyces cerevisiae (yeast) supplementation on varied cereal-based broiler starter diets on gut microflora and morphology. J Sci Res and Reports 6 (1): 73-81.
  30. Rezende M.S., a.v. Mundim, B.B. Fonseca, R.l. Miranda, W. Oliveira Jr and C.G.Lelli. 2017 Profile of serum metabolites and proteins of broiler breeders in rearing age. Brazilian Journal of Poultry Science 19 (4): 583-586.
  31. Shang Y., S. Kumar, B. Oakley, W.K. Kim. 2018 Chicken gut microbiota: importance and detection technology. Front Vet Sci 5: 254. Doi:10.3389/fvets.2018.00254.
  32. Tang S.G.H., C.C. Sieo, K.Ramasamy, W.Z. Saad, H.K.Wong and Y.W. Ho. 2017 Peroformance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with probiotic, probiotic and symbiotic. BMC Veterinary Research 13: 248-259.
  33. Toghyani M., M. Toghyani, S.A. Tabeidian. 2011 Effect of probiotic and antibiotic growth promoter substitutions on productive and carcass traits of broiler chicks. International Conference of Food Engineering and Biotechnology IPCBEF 9, Singapore.
  34. Williams B.J.. 1971 Factors which influence synergism by neomycin and oxytetracycline. Appl Microbiol 21 (4): 668-672.
  35. Wistrand-Yuen E., M. Knopp, K. Hjort, S. Koskiniemi, O.G. Berg, and D.I. Andersson. 2018. Evolution of high-level resistance during low-level antibiotic exposure. Nat Com 9: 1599. Doi: 10.1038/s41467-018-04059-1.
  36. Yadav S., R. Jha. 2019. Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J Anim Sci and Biotech 10 (2): 1-11.