Analysis of the Nutritional Content, Fiber Fractions, and Methane Emissions of Lemongrass Waste Feed Produced Through Fermentation Using White Rot Fungi
DOI:
https://doi.org/10.20884/1.jap....510Keywords:
Fermentation, lemongrass, white rot fungus, fiber fraction, methane emissionsAbstract
This study was conducted to examine the effectiveness of Trametes versicolor, Phanerochaete chrysosporium, Lentinula edodes, and a consortium (Trametes versicolor + Phanerochaete chrysosporium) in enhancing the nutrient content and reducing the fiber fraction of fermented lemongrass waste. This study was conducted at the Faculty of Agriculture, Syiah Kuala University. Using the completely randomized design (CRD) with 5 treatments and 4 replicates, this study examined dry matter, crude protein, crude fiber, crude fat, ash, NFE, ADF, NDF, cellulose, hemicellulose, lignin, and methane emissions. The data were analyzed using ANOVA, followed by Duncan’s multiple range test. This study concludes there is a numerical trend toward higher crude protein content in treatment P4 (Consortium; 5.51 ± 1.26) compared with the control (4.12 ± 0.32); however, the difference was not statistically significant (p = 0.06). However, in the fiber fraction analysis, the NDF content was lowest in the control group (59.68±0.34) compared to treatments P1, P2, P3, and P4. The control treatment exhibited the highest methane emission (7.1 × 10⁴ ppm), whereas the consortium treatment (P4) produced the lowest methane emission (2.96 × 10⁴ ppm). ANOVA revealed a significant effect of treatment on methane emissions (p = 0.009), and Duncan's multiple range test confirmed that the consortium treatment differed significantly from the control. The greatest reduction in the consortium treatment indicates a synergistic effect among the fungi in modifying the substrate, thereby more effectively suppressing methane formation.
Downloads
References
Adesogan AT, KG Arriola, Y Jiang, A Oyebade, EM Paula, A Pech-Cervantes, JJ Romero, L Ferraretto, and D Vyas. 2019. Technologies for improving fiber utilization. Journal of Dairy Science, 102(6), 5726–5755. https://doi.org/10.3168/jds.2018-15334
Anggara M, I Munandar, SF Utami, FD Ikram, and M Faisal. 2022. Manajemen pemeliharaan dan pemberian pakan ternak sapi potong di Desa Sebewe Kecamatan Moyo Utara, Kabupaten Sumbawa. JASTEN (Jurnal Aplikasi Sains Teknologi Nasional), 3(2), 64–68. https://doi.org/10.36040/jasten.v3i2.5910
Astudillo-Neira R, S Suescun-Ospina, N Vera-Aguilera, J Alarcon-Enos, and J Ávila-Stagno. 2023. Biodegraded hay with graded addition of Pleurotus ostreatus improves dry matter disappearance and reduces methane production of diets incubated in vitro. Italian Journal of Animal Science, 22(1), 347–358. https://doi.org/10.1080/1828051X.2023.2193607
Astuti T, SA Akbar, and F Basyirun. 2024. Limbah serai wangi sebagai pakan ternak ruminansia. 1st ed. PT Insan Cendekia Mandiri Group, Sumatra Barat.
Carrillo-Díaz MI, O Arce-Cervantes, GD Mendoza-Martínez, LA Miranda-Romero, PA Hernández-García, and R Rojo-Rubio. 2022. Improvement of ruminal neutral detergent fiber degradability by obtaining and using exogenous fibrolytic enzymes from white-rot fungi. Animals, 12(7), 843. https://doi.org/10.3390/ani12070843
Chen J, X Ma, M Liang, Z Guo, Y Cai, C Zhu, Z Wang, S Wang, J Xu, and H Ying. 2024. Physical–chemical–biological pretreatment for biomass degradation and industrial applications: A review. Waste, 2(4), 451–473. https://doi.org/10.3390/waste2040024
Daniso E, and M Spanghero. 2026. Ligninolytic enzyme content of mushroom Pleurotus ostreatus and Agaricus bisporus extracts and in vitro rumen fermentation of fungal extract-treated wheat straw. Food Science and Nutrition, 14(3), e71471. https://doi.org/10.1002/fsn3.71471
Elisashvili V, M Penninckx, E Kachlishvili, N Tsiklauri, E Metreveli, T Kharziani, and G Kvesitadze. 2008. Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition. Bioresource Technology, 99(3), 457–462. https://doi.org/10.1016/j.biortech.2007.01.011
Kamid RAA, L Khotijah, and NR Kumalasari. 2024. Analisis keragaman kualitas nutrien berbagai pakan ruminansia di wilayah Indonesia. Jurnal Ilmu Nutrisi dan Teknologi Pakan, 22(1), 14–22. https://doi.org/10.29244/jintp.22.1.14-22
Khan NA, M Khan, A Sufyan, A Saeed, L Sun, S Wang, M Nazar, Z Tan, Y Liu, and S Tang. 2024. Biotechnological processing of sugarcane bagasse through solid-state fermentation with white rot fungi into nutritionally rich and digestible ruminant feed. Fermentation, 10(4), 181. https://doi.org/10.3390/fermentation10040181
Kholif AE, GA Gouda, and AK Patra. 2022. The sustainable mitigation of in vitro ruminal biogas emissions by ensiling date palm leaves and rice straw with lactic acid bacteria and Pleurotus ostreatus for cleaner livestock production. Journal of Applied Microbiology, 132(4), 2925–2939. https://doi.org/10.1111/jam.15432
Kumar S, SS Dagar, AK Puniya, and RC Upadhyay. 2013. Changes in methane emission, rumen fermentation in response to diet and microbial interactions. Research in Veterinary Science, 94(2), 263–268. https://doi.org/10.1016/j.rvsc.2012.09.007
McDonald P, RA Edwards, JFD Greenhalgh, CA Morgan, LA Sinclair, and RG Wilkinson. 2022. Animal nutrition. 9th ed. Pearson Education Limited, Harlow.
Olagunju LK, OS Isikhuemhen, PA Dele, FN Anike, JO Alabi, KA Ike, Y Shaw, RM Brice, OE Orimaye, M Wuaku, NS Udombang, and UY Anele. 2024. The impact of three white-rot fungi on nutrient availability, greenhouse gas emissions, and volatile fatty acid production in myceliated sorghum. Foods, 13(14), 2199. https://doi.org/10.3390/foods13142199
Pratama SM, M Jamilah, SS Dewi, Halifah, M Hamzah, H Koesmara, and M Ammar. 2025. Utilization of fermented coffee pulp using Trichoderma viride with different colony forming unit on in vitro digestibility as alternative forage. IOP Conference Series: Earth and Environmental Science, 1616(1), 012025. https://doi.org/10.1088/1755-1315/1616/1/012025
Pratama SM, S Wajizah, A Jayanegara, and Samadi. 2022. Evaluation of some forage as feed for ruminant animal: Chemical composition, in vitro rumen fermentation, and methane emissions. Animal Production, 24(3), 150–160. https://doi.org/10.20884/1.jap.2022.24.3.179
Qi J, F Li, L Jia, X Zhang, S Deng, B Luo, Y Zhou, M Fan, and Y Xia. 2023. Fungal selectivity and biodegradation effects by white and brown rot fungi for wood biomass pretreatment. Polymers, 15(8), 1957. https://doi.org/10.3390/polym15081957
Samadi, I Wahyudi, S Wajizah, Zulfahrizal, and AA Munawar. 2024. Robust near infrared spectroscopy for rapid and simultaneous determination of fermented cocoa pod husk feed quality attributes. International Journal of Design and Nature and Ecodynamics, 19(2), 379–386. https://doi.org/10.18280/ijdne.190203
Sanchez C. 2009. Lignocellulosic residues: Biodegradation and bioconversion by fungi. Biotechnology Advances, 27(2), 185–194. https://doi.org/10.1016/j.biotechadv.2008.11.001
Sanchez C. 2020. White-rot fungi and their enzymes for the treatment of lignocellulosic biomass: Biological pretreatment and biotechnological applications. Biomolecules, 10(9), 1269. https://doi.org/10.3390/biom10091269
Septian MH, I Hernaman, R Wiradimadja, and FT Santoso. 2018. Performance and diet digestibility of male Garut lamb fed Ipomea reptans seed. Buletin Peternakan, 42(4), 278–282. https://doi.org/10.21059/buletinpeternak.v42i4.31647
Sukamto, and M Djazuli. 2011. Limbah serai wangi potensial sebagai pakan ternak. Warta Penelitian dan Pengembangan Pertanian, 33(6), 10–12.
Tuyen DV, HN Phuong, JW Cone, JJP Baars, ASM Sonnenberg, and WH Hendriks. 2013. Effect of fungal treatments of fibrous agricultural by-products on chemical composition and in vitro rumen fermentation and methane production. Bioresource Technology, 129, 256–263. https://doi.org/10.1016/j.biortech.2012.10.128
van Kuijk SJA, ASM Sonnenberg, JJP Baars, WH Hendriks, and JW Cone. 2015. Fungal treated lignocellulosic biomass as ruminant feed ingredient: A review. Animal Feed Science and Technology, 202, 17–34. https://doi.org/10.1016/j.anifeedsci.2015.02.001
Wajizah S, FA Fhonna, F Khairi, and M Delima. 2022. In vitro rumen fermentation characteristics of fermented complete feed based on rice straw with moringa leaves (Moringa oleifera) addition. IOP Conference Series: Earth and Environmental Science, 1116(1), 012044. https://doi.org/10.1088/1755-1315/1116/1/012044
Wu Z, K Peng, Y Zhang, M Wang, C Yong, L Chen, P Qu, H Huang, E Sun, and M Pan. 2022. Lignocellulose dissociation with biological pretreatment towards the biochemical platform: A review. Materials Today Bio, 16, 100445. https://doi.org/10.1016/j.mtbio.2022.100445
Downloads
Published
Issue
Section
License
Submission of a manuscript implies that the work described has not been published before or is under consideration for publication elsewhere (except in the form of an abstract). When the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.


