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Abstract

Long-term use of antibiotics in broiler chicken farming causes the emergence of resistant bacteria and increases the potential for multidrug-resistant bacteria. This study aims to investigate the presence and characteristics of extended-spectrum β-lactamase (ESBL) producing Escherichia coli (E. coli) in broiler farms located in Ciampea, Bogor. A total of 90 samples were taken from three broiler chicken farms located in Ciampea, Bogor. Sampling was carried out by swabbing the cloaca of 1% of the population on each farm. Gram staining and IMVIC biochemical tests were performed on colonies suspected of being E. coli. Confirmation of ESBL-producing E. coli using a double disc diffusion test with MHA. The results showed that ESBL-producing E. coli was present in 28,9% of broiler chicken cloaca swab samples (26/90). Antibiotic resistance tests of ESBL-producing E. coli isolates showed resistance to four antibiotics, namely chloramphenicol, amoxicillin, ciprofloxacin, and ampicillin. In this test, 22 isolates showed multidrug-resistant

Keywords

Escherichia coli ESBL Broiler Antibiotics Bacteria

Article Details

How to Cite
Putra, K., Wahyuningsih, W., Purwanti, M., Handayani, K. S., & Julaeha, J. (2025). Extended-spectrum β-lactamase Producing Escherichia coli in Broiler Farms in Ciampea Bogor. ANIMAL PRODUCTION, 27(1), 11-17. https://doi.org/10.20884/1.jap.2025.27.1.299

References

  1. Ayinla AO, and ALP Mateus. 2023. Extended-spectrum beta-lactamases in poultry in Africa: a systematic review. Frontiers in Antibiotics. 2: 1140750.
  2. Bavadharani M, ST Rai, AK Arora, and S Singh. 2023. Antimicrobial sensitivity pattern and detection of antimicrobial resistance genes of E. coli isolated from respiratory tract infections in poultry. The Indian Journal of Animal Sciences., 93(3): 251–255.
  3. Castanheira M , PJ Simner, and PA Bradford. 2021. Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection. JAC-Antimicrobial Resistance. 3(3)
  4. Clinical and Laboratory Standards Institute (CLSI). 2021. CLSI M100-ED31:2021 Performance Standards for Antimicrobial Susceptibility Testing, 31st Edition.
  5. Hermana NSP, Z Nilasari, E Yuniarti, A Riyanto, Safika, A Indrawati, FH Pasaribu. Antibiotic resistance profile of Staphylococcus aureus, Streptococcus spp. and Klebsiella spp. isolated form chicken farm in Bogor, Sukabumi, and Cianjur. Journal of Physics: Conference Series. West Java. 1430: 1-6
  6. Kpoda DS, A Ajayi, M Somda, O Traore, N Guessennd. 2018. Distribution of resistance genes encoding ESBLs in Enterobacteriaceae isolated from biological samples in health centers in Ouagadougou, Burkina Faso. BMC Research Notes. 11:471.
  7. Lopatkin AJ, HR Meredith, JK Srimani, P Pfeiffer, R Durrett, and L You . 2022. Persistence and reversal of plasmid-mediated antibiotic resistance. Nature Communications. 8(1):1689.
  8. Mu’arofah B, RY Arizandy,B Utomo, and Kuntaman K. 2020. Detection Of Extended Spectrum Β-Lactamase (ESBL) Gene Patterns Of Enterobacteriaceae In Broiler Chicken Meat Sold In Traditional Markets In The East Surabaya. Jurnal Ilmiah Kesehatan. 9 (1); 12-19. doi: 10.30994/sjik.v9i1.264
  9. Mueller M, and CR Tainter. 2022. Escherichia coli infection. StatPearls. Treasure Island (FL): StatPearls Publishing. PMID: 33231968.
  10. Müller A, R Stephan, Magdalena, and Nüesch-Inderbinen . 2015. Distribution of virulence factors in ESBL-producing Escherichia coli isolated from the environment, livestock, food and humans. Science of The Total Environment. 541:667-672.
  11. Naelasari DN, EB Koendhori, L Dewanti, R Sarassari, and K Kuntaman. 2018. The prevalence of extended spectrum beta-lactamase (ESBL) producing gut bacterial flora among patients in Dr. Soetomo hospital and primary health centre in Surabaya. Folia Medica Indonesiana. 54(4): 256-262.
  12. Rawat D, and D Nair. 2010. Extended-spectrum β-lactamases in gram negative bacteria. Journal of Global Infectious Diseases. . 2(3):263-74.
  13. Rousham EK, L Unicomb, and MA Islam. 2018. Human, animal and environmental contributors to antibiotic resistance in low-resource settings: integrating behavioural, epidemiological and one health approaches. Proceedings: Biological Sciences. . 285(1876): 20180332.
  14. Rumokoy, L, and WL Toar. 2022. Application of Thoracic Immunogen of Musca domestica on Immunoglobulin-G Level of Goats Detected Through a Single Radial Immuno-Diffusion Test. Animal Production. 24(1):1–5.
  15. Schaufler K, Bethe A, Lübke-Becker A, Ewers C, Kohn B, Wieler L. H, Geunther S. 2015. Putative connection between zoonotic multi resistant extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in dog feces from a veterinary campus and clinical isolates from dogs. Infection Ecology & Epidemiology. 5(4):25334-25339.
  16. Umadevi S, Kandhakumari G, Joseph NM, Kumar S, Easow JM, Stephen S .2011. Prevalence and antimicrobial susceptibility pattern of ESBL-producing gram-negative bacilli. Journal of Clinical and Diagnostic Research. 5:236-239.
  17. van Hoek AHAM, C Dierikx, T Bosch, L Schouls , E van Duijkeren, and M Visser. 2020. Transmission of ESBL-producing Escherichia coli between broilers and humans on broiler farms. Journal of Antimicrobial Chemotherapy. 75: 543–549.
  18. Ventola CL. 2015. The antibiotic resistance crisis: part 1-causes and threats. Pharmacy Therapeutics. 40(4):277-83. PMCID: PMC4378521
  19. [WHO] World Health Organization. 2018. Antimicrobial resistance. In: facts sheet
  20. [WHO] World Health Organization. 2021. WHO integrated global surveillance on ESBL-producing E. coli using a “One health” approach.
  21. Wibisono FJ, B Sumiarto, T Untari, MH Effendi, DA Permatasari, and AM Witaningrum. 2020. CTX Gene of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli on Broilers in Blitar, Indonesia. Scientific Research Publishing. . 11(7): 396-403.
  22. Widhi APKN, and INY Saputra. 2021. Broiler Chicken Meat Sold at Purwokerto's Market Has Antibiotic Residues and Escherichia coli That Produces ESBL. J. Kesehatan Lingkungan Indonesia. 20(2):137-142